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Approach to Identifying Disease-Modifying Genes
Animal Model Approach
One study examined myocardial gene expression responses to CPB in the absence of cardioplegic arrest and ischemia-reperfusion injury using a unique rat model.
Sternotomy, direct operations on the heart, aortic crossclamping, and cardioplegic arrest were not performed. Hearts from 6 animals randomized to either 90 minutes of CPB or sham control animals were used to perform cDNA microarray analyses of 2343 genes. Real-time quantitative polymerase chain reaction was used to confirm the microarray results for a subset of genes.
42 differentially genes were expressed. They include the transcription activator nuclear factor kappaB, adhesion molecules (vascular cell adhesion molecule 1 and P-selectin), and interleukin 6 receptor subunits; downregulated genes include transforming growth factor beta receptor 2, tissue inhibitor of metalloproteinase 3, and mitogen-activated protein kinase 1.
Podgoreanu et al, J Thorac Cardiovasc Surg 2005
Find Out More:
- 42 differentially expressed genes
- Pro-inflammatory genes over-represented
Podgoreanu et al, J Thorac Cardiovasc Surg 2005
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